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KMID : 0368420190620050351
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Journal of Plant Biology
2019 Volume.62 No. 5 p.351 ~ p.358
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Analysis of Tissue-specific RNA Editing Events of Genes Involved in RNA Editing in Arabidopsis thaliana
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Qulsum Umme
Azad Md. Thoufic Anam
Tsukahara Toshifumi
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Abstract
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In plants, RNA editing is a post-transcriptional modification of transcripts and commonly occurs in plastids and mitochondria. In the case of flowering plants, not only PPR but also non-PPR proteins, such as MORF/RIP, ORRM and OZ partake in the diverse RNA editing complex. In Arabidopsis thaliana, 12 types of RNA editing patterns have been predicted in nuclear, chloroplast, and mitochondrial transcripts. In this study, tissue-specific RNA editing events were detected in gene families involved in RNA editing. Different Arabidopsis tissues at variable developmental stages, including 4-, 8-, and 12-d-old seedlings and 16-, 21-, 27-, and 32-d-old leaves, stems, stipes and roots, were collected and used for this study. Nine types of RNA editing events, including C-to-U, U-to-C, A-to-I, A-to-C, A-to-U, G-to-A, G-to-C, U-to-A and U-to-G, were identified in target genes. Most of the editing events occurred in seedlings and leaves and a few in stem tissues. Extensive U-to-C editing (60%) and A-to-G editing (54%) was detected in 12-d-old seedlings and 21-d-old leaves, respectively. This is the first experimental report of tissue-specific nuclear RNA editing events found in plants. This study will provide important information for revealing the mechanism of RNA editing in plants.
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KEYWORD
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Arabidopsis thaliana, RNA editing events, Tissue-specific
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